Journal: Infection and Immunity

Article Title: Thioredoxin from Brugia malayi: Defining a 16-Kilodalton Class of Thioredoxins from Nematodes

doi: 10.1128/IAI.71.7.4119-4126.2003

Figure Lengend Snippet: Bm-TRX-1 inhibits activation of p38 MAP kinase. The WCPPCR and WCPQCR alleles of Bm-trx-1 were cloned separately into a eukaryotic expression plasmid vector, and the plasmids were introduced into TPA-differentiated ML-1 cells. The transfected ML-1 cells were stimulated with TNF-α (100 IU/well) for 5 min, and the phosphorylation status of p38 MAP kinase was analyzed on immunostained Western blots of cell extracts. Controls included cells maintained in medium (medium control), cells exposed to the transfection regent (transfection control), and cells transfected with a plasmid containing no insert (plasmid control).

Article Snippet: In preliminary experiments, TPA-differentiated ML-1 cells were stimulated with TNF-α (100 IU/well) for 5, 10, and 30 min, and the phosphorylation status of p38 MAP kinase was analyzed on immunostained Western blots of cell extracts with phosphoPlus p38 MAP kinase antibody kit (Cell Signaling Technology, Beverly, Mass.).

Techniques: Activation Assay, Clone Assay, Expressing, Plasmid Preparation, Transfection, Western Blot

Journal: Nature

Article Title: Neutrophil ageing is regulated by the microbiome

doi: 10.1038/nature15367

Figure Lengend Snippet: Pathways selected for the analysis of neutrophil functions.

Article Snippet: MAPK pathway , BIOCARTA_MAPK_PATHWAY.

Techniques: Coagulation, Activation Assay, Ubiquitin Proteomics

Journal: Nature

Article Title: Neutrophil ageing is regulated by the microbiome

doi: 10.1038/nature15367

Figure Lengend Snippet: Gene set enrichment analysis of selected pathways in aged and activated neutrophils.

Article Snippet: MAPK pathway , BIOCARTA_MAPK_PATHWAY.

Techniques: Coagulation, Activation Assay, Ubiquitin Proteomics

Journal: Biochemical Journal

Article Title: The myeloperoxidase-derived oxidant HOSCN inhibits protein tyrosine phosphatases and modulates cell signalling via the mitogen-activated protein kinase (MAPK) pathway in macrophages

doi: 10.1042/BJ20100082

Figure Lengend Snippet: MAPK gene array following treatment of J774A.1 cells with HOSCN. Cells (plated at 0.5×10 6 ml −1 ) were exposed to 50 μM HOSCN (open bars) or 100 μM HOSCN (solid bars) in PBS at 37 °C for 15 min. Total RNA was subsequently extracted, used to synthesize cDNA and used in the MAPK signalling pathway PCR array as described in the Experimental section. Histograms show data for: ( A ) transcription factors, ( B ) cyclin-related proteins, ( C ) MAPKs and ( D ) other genes. All genes were normalized against five reference genes and data are expressed relative to control (PBS-treated) cells, and are means±S.E.M. for triplicate determinations from three independent experiments. Statistical analyses compared the HOSCN-treated samples with the no-oxidant (PBS-treated) control cells using one-way ANOVA with Bonferroni's post-hoc test, * P <0.05.

Article Snippet: The resultant cDNA was assayed by PCR amplification using a MAPK Signalling Pathway PCR Array kit (SA Biosciences) based on the RT 2 SYBR green/Fluorescein PCR master mix (SA Biosciences).

Techniques: